Post by kickingfrog on Apr 1, 2012 15:13:35 GMT
Gut, 1985, 26, 169-174
Acute gluten challenge in treated adult coeliac disease:
a morphometric and enzymatic study
M G BRAMBLE, S ZUCOLOTO, N A WRIGHT, AND C 0 RECORD
From the Gastroenterology Unit, Royal Victoria Infirmary and University ofNewcastle upon Tyne and
Department of Histopathology, Royal Postgraduate Medical School Hammersmith Hospital, London
SUMMARY
Using a Quinton hydraulic biopsy tube, jejunal biopsies were obtained from 10
patients with adult coeliac disease in remission and four healthy volunteers before and after
administration of gluten fraction III into the proximal duodenum.
The biopsies taken at hourly
intervals for four hours, were analysed for changes in brush border enzymes, light microscopic
appearances, and villous and crypt population counts.
The results indicate that mucosal damage
occurs within three to four hours of gluten administration with significant falls in brush border
enzyme concentrations and villous population counts.
The absence of any change in control
biopsies indicates that gluten sensitivity is specific to the mucosa of patients with coeliac disease,
the timing of the changes being consistent with a type III immune response or direct toxicity.
Some recovery of the brush border enzymes but not the villous population was evident 24 hours
after gluten administration while the crypt population showed evidence of a compensatory
hyperplastic reaction.
gut.bmj.com/content/26/2/169.full.pdf
**************
Acute gluten challenge in treated adult coeliac disease:
a morphometric and enzymatic study
M G BRAMBLE, S ZUCOLOTO, N A WRIGHT, AND C 0 RECORD
From the Gastroenterology Unit, Royal Victoria Infirmary and University ofNewcastle upon Tyne and
Department of Histopathology, Royal Postgraduate Medical School Hammersmith Hospital, London
SUMMARY
Using a Quinton hydraulic biopsy tube, jejunal biopsies were obtained from 10
patients with adult coeliac disease in remission and four healthy volunteers before and after
administration of gluten fraction III into the proximal duodenum.
The biopsies taken at hourly
intervals for four hours, were analysed for changes in brush border enzymes, light microscopic
appearances, and villous and crypt population counts.
The results indicate that mucosal damage
occurs within three to four hours of gluten administration with significant falls in brush border
enzyme concentrations and villous population counts.
The absence of any change in control
biopsies indicates that gluten sensitivity is specific to the mucosa of patients with coeliac disease,
the timing of the changes being consistent with a type III immune response or direct toxicity.
Some recovery of the brush border enzymes but not the villous population was evident 24 hours
after gluten administration while the crypt population showed evidence of a compensatory
hyperplastic reaction.
gut.bmj.com/content/26/2/169.full.pdf
**************